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Science 24 September 1982:
Vol. 217. no. 4566, pp. 1252 - 1255
DOI: 10.1126/science.7112126
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Articles

Science, Vol 217, Issue 4566, 1252-1255
Copyright © 1982 by American Association for the Advancement of Science

articles

Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate

H Giloh and JW Sedat

n-Prop]yl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.


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