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Science 17 September 1982:
Vol. 217. no. 4565, pp. 1147 - 1149
DOI: 10.1126/science.217.4565.1147
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Articles

Cell-Free Circularization of Viroid Progeny RNA by an RNA Ligase from Wheat Germ

ANDREA D. BRANCH 1, HUGH D. ROBERTSON 1, CHRISTOPHER GREER 2, PETER GEGENHEIMER 2, CRAIG PEEBLES 2, and JOHN ABELSON 2

1 Laboratory of Genetics, Rockefeller University, New York 10021
2 Department of Chemistry, University of California, San Diego, La Jolla, 92093

Linear, potato spindle tuber viroid RNA has been used as a substrate for an RNA ligase purified from wheat germ. Linear viroid molecules are efficiently converted to circular molecules (circles) which are indistinguishable by electrophoretic mobility and two-dimensional oligonucleotide pattern from viroid circles extracted from infected plants. In light of recent evidence for multimeric viroid replication intermediates, cleavage followed by RNA ligation by a cellular enzyme may (i) be a normal step in the viroid life cycle and (ii) may also reflect cellular events.

Submitted on March 12, 1982
Revised on June 25, 1982


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Monomeric Linear RNA of Citrus Exocortis Viroid Resulting from Processing In Vivo Has 5'-Phosphomonoester and 3'-Hydroxyl Termini: Implications for the RNase and RNA Ligase Involved in Replication.
M.-E. Gas, D. Molina-Serrano, C. Hernandez, R. Flores, and J.-A. Daros (2008)
J. Virol. 82, 10321-10325
   Abstract »    Full Text »    PDF »
Arabidopsis thaliana has the enzymatic machinery for replicating representative viroid species of the family Pospiviroidae.
J.-A. Daros and R. Flores (2004)
PNAS 101, 6792-6797
   Abstract »    Full Text »    PDF »
Detection of tRNA-like Structure through RNase P Cleavage of Viral Internal Ribosome Entry Site RNAs Near the AUG Start Triplet.
A. J. Lyons and H. D. Robertson (2003)
J. Biol. Chem. 278, 26844-26850
   Abstract »    Full Text »    PDF »
Natural 2',5'-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid.
F. Côté, D. Lévesque, and J.-P. Perreault (2001)
J. Virol. 75, 19-25
   Abstract »    Full Text »
Characterization of the Escherichia coli RNA 3'-Terminal Phosphate Cyclase and Its sigma 54-Regulated Operon.
P. Genschik, K. Drabikowski, and W. Filipowicz (1998)
J. Biol. Chem. 273, 25516-25526
   Abstract »    Full Text »    PDF »
A replication cycle for viroids and other small infectious RNA's.
A. Branch and H. Robertson (1984)
Science 223, 450-455
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)