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Science 3 September 1982:
Vol. 217. no. 4563, pp. 953 - 955
DOI: 10.1126/science.7112109
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Articles

Science, Vol 217, Issue 4563, 953-955
Copyright © 1982 by American Association for the Advancement of Science

articles

Neuronal mapping: a photooxidation reaction makes Lucifer yellow useful for electron microscopy

AR Maranto

Irradiation Lucifer yellow-filled neurons with intense blue light in the presence of 3,3'-diaminobenzidine produces an electron-opaque osmiophilic polymer within the injected cells. This technique is valuable when cobalt or horseradish peroxidase injections are difficult or when a second intracellular marker is needed to demonstrate neuronal contacts.


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Multicolor and Electron Microscopic Imaging of Connexin Trafficking.
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Phalloidin-Eosin Followed by Photo-oxidation: A Novel Method for Localizing F-Actin at the Light and Electron Microscopic Levels.
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Correlative Fluorescence and Electron Microscopy on Ultrathin Cryosections: Bridging the Resolution Gap.
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Correlative Microscopy Using FluoroNanogold on Ultrathin Cryosections: Proof of Principle.
T. Takizawa, K. Suzuki, and J. M. Robinson (1998)
J. Histochem. Cytochem. 46, 1097-1102
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Electron Microscopic Visualization of Receptor–mediated Endocytosis of DiI–labeled Lipoproteins by Diaminobenzidine Photoconversion.
N. P. Dantuma, M. A. P. Pijnenburg, J. H. B. Diederen, and D. J. Van der Horst (1998)
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A Rapid Method for Combined Laser Scanning Confocal Microscopic and Electron Microscopic Visualization of Biocytin or Neurobiotin-labeled Neurons.
X. J. Sun, L. P. Tolbert, J. G. Hildebrand, and I. A. Meinertzhagen (1998)
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Efficient Immunocytochemical Labeling of Leukocyte Microtubules with FluoroNanogold: An Important Tool for Correlative Microscopy.
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Subplate neurons pioneer the first axon pathway from the cerebral cortex.
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