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Science 27 August 1982:
Vol. 217. no. 4562, pp. 843 - 845
DOI: 10.1126/science.7100928
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Articles

Science, Vol 217, Issue 4562, 843-845
Copyright © 1982 by American Association for the Advancement of Science

articles

High-affinity choline transport in proteoliposomes derived from rat cortical synaptosomes

EM Meyer and Cooper JR

Functional high- and low-affinity choline transport processes from rat cortical plasma membranes were reconstituted in phosphatidylcholine bilayer liposomes. The high-affinity choline transporter demonstrated a pharmacological profile and ion dependency that were identical to those of intact synaptosomes. This preparation may be used to further characterize choline transport and, with appropriate supplementation, to investigate the release of acetylcholine in the absence of synaptic vesicles.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)