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Science 13 August 1982:
Vol. 217. no. 4560, pp. 648 - 650
DOI: 10.1126/science.6283640
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Articles

Science, Vol 217, Issue 4560, 648-650
Copyright © 1982 by American Association for the Advancement of Science

articles

Active genes are sensitive to deoxyribonuclease I during metaphase

B Gazit, H Cedar, I Lerer, and R Voss

The active exogenous murine leukemia virus sequences of mouse cells growing in culture are preferentially digested by deoxyribonuclease I in metaphase chromosomes. As determined by nuclear nick translation, all of the gene sequences of these cells active during interphase are in a deoxyribonuclease I-sensitive conformation during metaphase. This method of nick translation can therefore be used to label chromosomes in situ in order to visualize the active regions of the genome.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mitotic Phosphorylation of Chromosomal Protein HMGN1 Inhibits Nuclear Import and Promotes Interaction with 14.3.3 Proteins.
M. Prymakowska-Bosak, R. Hock, F. Catez, J.-H. Lim, Y. Birger, H. Shirakawa, K. Lee, and M. Bustin (2002)
Mol. Cell. Biol. 22, 6809-6819
   Abstract »    Full Text »    PDF »
Nonrandom Degradation of DNA in Human Leukemic Cells during Radiation-induced Apoptosis.
R. G. Dullea, J. F. Robinson, and J. S. Bedford (1999)
Cancer Res. 59, 3712-3718
   Abstract »    Full Text »    PDF »


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