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Science 16 July 1982:
Vol. 217. no. 4556, pp. 252 - 254
DOI: 10.1126/science.6806904
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Articles

Science, Vol 217, Issue 4556, 252-254
Copyright © 1982 by American Association for the Advancement of Science

articles

Measurement of intracellular free calcium in monkey kidney cells with aequorin

AB Borle and KW Snowdowne

A method has been developed for the measurement of intracellular free calcium in mammalian cells. The calcium-sensitive photoprotein aequorin can be incorporated into isolated cells by hypo-osmotic treatment without altering the cell viability, permeability, or metabolism. Intracellular calcium activity (Cai2+) was monitored in a perfusion system. In monkey kidney cells (LLC-MK2), Cai2+ is approximately 57 nanomoles per liter. Changes in Cai2+ with time can also be followed: exposure of the cells to anaerobiosis or the calcium ionophore A23187 reversibly increases Cai2+. The method has also been successfully tested in rat hepatocytes.


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