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Science 2 July 1982:
Vol. 217. no. 4554, pp. 68 - 70
DOI: 10.1126/science.6806900
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Articles

Science, Vol 217, Issue 4554, 68-70
Copyright © 1982 by American Association for the Advancement of Science

articles

Autoradiographic identification of ornithine decarboxylase in mouse kidney by means of alpha-[5-14C]difluoromethylornithine

AE Pegg, J Seely, and IS Zagon

alpha-Difluoromethylornithine is an enzyme-activated irreversible inhibitor of ornithine decarboxylase that forms a covalent bond with the enzyme. When alpha-[5-14C]difluoromethylornithine was administered to androgen-treated mice, only ornithine decarboxylase became labeled. Autoradiographic examination of kidney, liver, and brain indicated much more extensive incorporation of labeled difluoromethylornithine into kidney protein than into the protein of the other tissues. Such incorporation was greatly reduced by prior treatment of the mice with cycloheximide. These results correlate with the presence of ornithine decarboxylase activity which is much higher in the kidney than in the other tissues and is lost rapidly when protein synthesis is inhibited. The binding of this drug in vivo, therefore, is useful for determining the distribution of ornithine decarboxylase. The enzyme was predominantly located in the proximal tubule cells of the kidney in androgen-treated mice.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Testosterone Down-Regulates Ornithine Aminotransferase Gene and Up-Regulates Arginase II and Ornithine Decarboxylase Genes for Polyamines Synthesis in the Murine Kidney.
O. Levillain, J.-J. Diaz, O. Blanchard, and H. Dechaud (2005)
Endocrinology 146, 950-959
   Abstract »    Full Text »    PDF »
Influence of testosterone on regulation of ODC, antizyme, and N1-SSAT gene expression in mouse kidney.
O. Levillain, A. Greco, J.-J. Diaz, R. Augier, A. Didier, K. Kindbeiter, F. Catez, and M. Cayre (2003)
Am J Physiol Renal Physiol 285, F498-F506
   Abstract »    Full Text »    PDF »
Distribution Patterns of Ornithine Decarboxylase in Cells and Tissues: Facts, Problems, and Postulates.
R. G. Schipper and A. A.J. Verhofstad (2002)
J. Histochem. Cytochem. 50, 1143-1160
   Abstract »    Full Text »    PDF »
Ornithine decarboxylase along the mouse and rat nephron.
O. Levillain and A. Hus-Citharel (1998)
Am J Physiol Renal Physiol 274, F1020-F1028
   Abstract »    Full Text »    PDF »


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