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Science 18 June 1982:
Vol. 216. no. 4552, pp. 1315 - 1317
DOI: 10.1126/science.216.4552.1315

Articles

Deep-Sea Bacteria: Isolation in the Absence of Decompression

HOLGER W. JANNASCH 1, CARL O. WIRSEN 1, and CRAIG D. TAYLOR 1

1 Biology Department, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543

Sampling and pure culture isolation of deep-sea bacteria without loss of in situ pressure is required in order to determine the viability of decompressionsensitive strains. This was achieved by using a pressure-retaining sterilizable seawater sampling system in connection with a prepressurized hyperbaric isolation chamber. Rates of growth and substrate uptake of the majority of isolates showed highly barotolerant characteristics, while the remainder (4 out of 15) exhibited barophilic characteristics.

Submitted on January 21, 1982


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Study of Deep-Sea Natural Microbial Populations and Barophilic Pure Cultures Using a High-Pressure Chemostat.
C. O. Wirsen and S. J. Molyneaux (1999)
Appl. Envir. Microbiol. 65, 5314-5321
   Abstract »    Full Text »
Death of a Hadal Deep-Sea Bacterium After Decompression.
A. A. YAYANOS and A. S. DIETZ (1983)
Science 220, 497-498
   Abstract »    PDF »



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Science. ISSN 0036-8075 (print), 1095-9203 (online)