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Articles
Deep-Sea Bacteria: Isolation in the Absence of Decompression
1 Biology Department, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543
Sampling and pure culture isolation of deep-sea bacteria without loss of in situ pressure is required in order to determine the viability of decompressionsensitive strains. This was achieved by using a pressure-retaining sterilizable seawater sampling system in connection with a prepressurized hyperbaric isolation chamber. Rates of growth and substrate uptake of the majority of isolates showed highly barotolerant characteristics, while the remainder (4 out of 15) exhibited barophilic characteristics.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)