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Science 14 May 1982:
Vol. 216. no. 4547, pp. 757 - 759
DOI: 10.1126/science.7079738
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Articles

Science, Vol 216, Issue 4547, 757-759
Copyright © 1982 by American Association for the Advancement of Science

articles

Lipofuscin: resolution of discrepant fluorescence data

GE Eldred, GV Miller, WS Stark, and L Feeney-Burns

Lipofuscin granules (age pigments) emit yellow light under ultraviolet excitation in the fluorescence microscope. The reported blue emission maximum of extracts of lipofuscin-laden cells may result from instrumental bias. The major fluorescent components that accumulate with age in these lysosomal residual bodies of human retinal pigment epithelium are yellow-emitting fluorophores. Different age-related fluorophores, which do emit blue light, are derived from other intracellular sources. A reevaluation of the connection between blue-emitting lipid peroxidation products and the age-related lipofuscin granules of classical pathology is necessary.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A2E, a Pigment of the Lipofuscin of Retinal Pigment Epithelial Cells, Is an Endogenous Ligand for Retinoic Acid Receptor.
A. Iriyama, R. Fujiki, Y. Inoue, H. Takahashi, Y. Tamaki, S. Takezawa, K. Takeyama, W.-D. Jang, S. Kato, and Y. Yanagi (2008)
J. Biol. Chem. 283, 11947-11953
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Photodistribution of Blue-Gray Hyperpigmentation After Amiodarone Treatment: Molecular Characterization of Amiodarone in the Skin.
A. Ammoury, S. Michaud, C. Paul, C. Prost-Squarcioni, F. Alvarez, L. Lamant, F. Launay, J. Bazex, N. Chouini-Lalanne, and M.-C. Marguery (2008)
Arch Dermatol 144, 92-96
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Spectral Profiling of Autofluorescence Associated with Lipofuscin, Bruch's Membrane, and Sub-RPE Deposits in Normal and AMD Eyes.
A. D. Marmorstein, L. Y. Marmorstein, H. Sakaguchi, and J. G. Hollyfield (2002)
Invest. Ophthalmol. Vis. Sci. 43, 2435-2441
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Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue.
S. A. Schnell, W. A. Staines, and M. W. Wessendorf (1999)
J. Histochem. Cytochem. 47, 719-730
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A role for vitamin A in the formation of ocular lipofuscin.
J. Wassell and M. Boulton (1997)
Br J Ophthalmol 81, 911-918
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