Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 May 1982:
Vol. 216. no. 4546, pp. 642 - 644
DOI: 10.1126/science.6280283
Prev | Table of Contents | Next

Articles

Science, Vol 216, Issue 4546, 642-644
Copyright © 1982 by American Association for the Advancement of Science

articles

Calmodulin binds to chick lens gap junction protein in a calcium-independent manner

MJ Welsh, JC Aster, M Ireland, J Alcala, and H Maisel

A biochemically active conjugate of calmodulin and tetramethylrhodamine isothiocyanate (CaM-RITC) was synthesized. When incubated with sections of chick lens, this conjugate bound to the surface membranes of lens fiber cells in the presence of absence of calcium. Incubation of lens sections with antibodies to gap junction protein of lens completely blocked the binding of the conjugate to cell membranes, whereas serum from nonimmunized animals or antibodies to others lens proteins reduced the binding only slightly. By means of a gel overlay procedure, 125I-labeled calmodulin was found to bind to the gap junction protein of lens, also in a calcium-independent manner. These results support the concept that calmodulin may interact with and regulate gap junctions in living cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Gap junctions and connexin-interacting proteins.
B. N.G Giepmans (2004)
Cardiovasc Res 62, 233-245
   Abstract »    Full Text »    PDF »
Calmodulin and protein kinase C regulate gap junctional coupling in lens epithelial cells.
M. M. Lurtz and C. F. Louis (2003)
Am J Physiol Cell Physiol 285, C1475-C1482
   Abstract »    Full Text »    PDF »
Sharing signals: connecting lung epithelial cells with gap junction channels.
M. Koval (2002)
Am J Physiol Lung Cell Mol Physiol 283, L875-L893
   Abstract »    Full Text »    PDF »
Myosin-1c Interacts with Hair-Cell Receptors through Its Calmodulin-Binding IQ Domains.
J. L. Cyr, R. A. Dumont, and P. G. Gillespie (2002)
J. Neurosci. 22, 2487-2495
   Abstract »    Full Text »    PDF »
Calmodulin activation of calcium-dependent sodium channels in excised membrane patches of Paramecium.
Y Saimi and K. Ling (1990)
Science 249, 1441-1444
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)