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Science 7 May 1982:
Vol. 216. no. 4546, pp. 581 - 590
DOI: 10.1126/science.6280281
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Articles

Science, Vol 216, Issue 4546, 581-590
Copyright © 1982 by American Association for the Advancement of Science

articles

DNA conformation, dynamics, and interactions in solution

DJ Patel, A Pardi, and K Itakura

The conformation and dynamics of the d(CGCGAATTCGCG) duplex, its analogs containing mismatched base pairs and helix interruptions, and its complexes with actinomycin and Netropsin, bound separately and simultaneously, have been investigated by nuclear magnetic resonance spectroscopy in aqueous solution. Structural information has been deduced from chemical shift and nuclear Overhauser effect parameters, while the kinetics have been probed from line width and saturation recovery experiments on proton and phosphorus markers at the individual base pair level. These studies lead to an improved understanding of the role of nucleic acid sequence on the structure, flexibility, and conformational interconversions in the duplex state. The nuclear magnetic resonance measurements readily identify helix modification and antibiotic binding sites on the nucleic acid and estimate the extent to which the observed conformational and dynamic perturbations are transmitted to adjacent base pair regions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Exocyclic amino groups of flanking guanines govern sequence-dependent adduct conformations and local structural distortions for minor groove-aligned benzo[a]pyrenyl-guanine lesions in a GG mutation hotspot context.
F. A. Rodriguez, Y. Cai, C. Lin, Y. Tang, A. Kolbanovskiy, S. Amin, D. J. Patel, S. Broyde, and N. E. Geacintov (2007)
Nucleic Acids Res. 35, 1555-1568
   Abstract »    Full Text »    PDF »
Mismatch-, MutS-, MutL-, and Helicase II-dependent Unwinding from the Single-strand Break of an Incised Heteroduplex.
V. Dao and P. Modrich (1998)
J. Biol. Chem. 273, 9202-9207
   Abstract »    Full Text »    PDF »
Design of sequence-specific DNA-binding molecules.
P. Dervan (1986)
Science 232, 464-471
   Abstract »    PDF »
Iron(II) EDTA used to measure the helical twist along any DNA molecule.
T. Tullius and B. Dombroski (1985)
Science 230, 679-681
   Abstract »    PDF »
NMR Studies of DNA Conformation and Dynamics in Solution.
D.J. Patel, S.A. Kozlowski, S. Ikuta, K. Itakura, R. Bhatt, and D.R. Hare (1983)
Cold Spring Harb Symp Quant Biol 47, 197-206
   Abstract »    PDF »
The Carbonate Radical Is a Site-selective Oxidizing Agent of Guanine in Double-stranded Oligonucleotides.
V. Shafirovich, A. Dourandin, W. Huang, and N. E. Geacintov (2001)
J. Biol. Chem. 276, 24621-24626
   Abstract »    Full Text »    PDF »


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