Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 2 April 1982:
Vol. 216. no. 4541, pp. 85 - 87
DOI: 10.1126/science.6121376
Prev | Table of Contents

Articles

Science, Vol 216, Issue 4541, 85-87
Copyright © 1982 by American Association for the Advancement of Science

articles

Dynorphin and vasopressin: common localization in magnocellular neurons

SJ Watson, H Akil, W Fischli, A Goldstein, E Zimmerman, G Nilaver, and TB van wimersma Griedanus

The opioid peptide dynorphin is widely distributed in neuronal tissue of rats. By immunocytochemical methods, it was shown previously that dynorphin-like immunoreactivity is present in the posterior pituitary and the cells of the hypothalamic neurosecretory magnocellular nuclei which also are responsible for the synthesis of oxytocin, vasopressin, and their neurophysins. By using an affinity-purified antiserum to the non-enkephalin part of the dynorphin molecule it has now been demonstrated that dynorphin and vasopressin occur in the same hypothalamic cells of rats, whereas dynorphin and oxytocin occur in separate cells. Homozygous Brattleboro rats (deficient in vasopressin) have magnocellular neurons that contain dynorphin separate from oxytocin. Thus dynorphin and vasopressin, although they occur in the same cells, appear to be under separate genetic control and presumably arise from different precursors.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Retrograde Opioid Signaling Regulates Glutamatergic Transmission in the Hypothalamus.
K. J. Iremonger and J. S. Bains (2009)
J. Neurosci. 29, 7349-7358
   Abstract »    Full Text »    PDF »
Feedback inhibition of action potential discharge by endogenous adenosine enhancement of the medium afterhyperpolarization.
M. Ruan and C. H. Brown (2009)
J. Physiol. 587, 1043-1056
   Abstract »    Full Text »    PDF »
Integration of Asynchronously Released Quanta Prolongs the Postsynaptic Spike Window.
K. J. Iremonger and J. S. Bains (2007)
J. Neurosci. 27, 6684-6691
   Abstract »    Full Text »    PDF »
Differential Cardiovascular and Renal Responses Produced by Microinjection of the {kappa}-Opioid U-50488H [(trans-3,4-Dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl]-benzene-acetamide) Methane Sulfonate] into Subregions of the Paraventricular Nucleus.
H. B. Gottlieb, K. J. Varner, V. A. Kenigs, A. M. Cabral, and D. R. Kapusta (2005)
J. Pharmacol. Exp. Ther. 312, 678-685
   Abstract »    Full Text »    PDF »
Burst Initiation and Termination in Phasic Vasopressin Cells of the Rat Supraoptic Nucleus: A Combined Mathematical, Electrical, and Calcium Fluorescence Study.
P. Roper, J. Callaway, and W. Armstrong (2004)
J. Neurosci. 24, 4818-4831
   Abstract »    Full Text »    PDF »
Gene Regulation in the Magnocellular Hypothalamo-Neurohypophysial System.
J. P. H. Burbach, S. M. Luckman, D. Murphy, and H. Gainer (2001)
Physiol Rev 81, 1197-1267
   Abstract »    Full Text »    PDF »
A Subset of Kappa Opioid Ligands Bind to the Membrane Glucocorticoid Receptor in an Amphibian Brain.
S. J. Evans, B. T. Searcy, and F. L. Moore (2000)
Endocrinology 141, 2294-2300
   Abstract »    Full Text »    PDF »
Stimulus-Dependent Translocation of kappa Opioid Receptors to the Plasma Membrane.
S. J. Shuster, M. Riedl, X. Li, L. Vulchanova, and R. Elde (1999)
J. Neurosci. 19, 2658-2664
   Abstract »    Full Text »    PDF »
kappa -Opioid Regulation of Neuronal Activity in the Rat Supraoptic Nucleus In Vivo.
C. H. Brown, M. Ludwig, and G. Leng (1998)
J. Neurosci. 18, 9480-9488
   Abstract »    Full Text »    PDF »
Dynorphin A1-13 Stimulates Ovine Fetal Pituitary-Adrenal Function through a Novel Nonopioid Mechanism.
C. C. Taylor, D. Wu, Y. Soong, J. S. Yee, and H. H. Szeto (1997)
J. Pharmacol. Exp. Ther. 280, 416-421
   Abstract »    Full Text »
The Concepts of Stress and Stress System Disorders: Overview of Physical and Behavioral Homeostasis.
G. P. Chrousos and P. W. Gold (1992)
JAMA 267, 1244-1252
   Abstract »    PDF »
Chronic Syndrome of Inappropriate Antidiuretic Hormone Secretion and Hypertension in a Patient With Olfactory Neuroblastoma: Evidence of Ectopic Production of Arginine Vasopressin by the Tumor.
J. Osterman, A. Calhoun, M. Dunham, U. X. Cullum Jr, R. M. Clark, D. D. Stewart, B. W. Scheithauer, E. A. Zimmerman, R. Defendini, X. Zang, et al. (1986)
Arch Intern Med 146, 1731-1735
   Abstract »    PDF »
Methionine and leucine enkephalin in rat neurohypophysis: different responses to osmotic stimuli and T2 toxin.
N Zamir, D Zamir, L. Eiden, M Palkovits, M. Brownstein, R. Eskay, E Weber, A. Faden, and G Feuerstein (1985)
Science 228, 606-608
   Abstract »    PDF »
Dynorphin-A-(1-8) is contained within vasopressin neurosecretory vesicles in rat pituitary.
M. Whitnall, H Gainer, B. Cox, and C. Molineaux (1983)
Science 222, 1137-1139
   Abstract »    PDF »
Immunoreactive dynorphin-(1-8) and corticotropin- releasing factor in subpopulation of hypothalamic neurons.
K. Roth, E Weber, J. Barchas, D Chang, and J. Chang (1983)
Science 219, 189-191
   Abstract »    PDF »
The Enkephalinergic Neuron: Implications of a Polyenkephalin Precursor.
J. Rossier, D. Liston, G. Patey, M. Chaminade, A.S. Foutz, A. Cupo, P. Giraud, M.P. Roisin, J.P. Henry, P. Verbanck, et al. (1983)
Cold Spring Harb Symp Quant Biol 48, 393-404
   Abstract »    PDF »
Comparison of the distribution of dynorphin systems and enkephalin systems in brain.
S. Watson, H Khachaturian, H Akil, D. Coy, and A Goldstein (1982)
Science 218, 1134-1136
   Abstract »    PDF »
The carboxy terminus of the precursor to vasopressin and neurophysin: immunocytochemistry in rat brain.
S. Watson, N. Seidah, and M Chretien (1982)
Science 217, 853-855
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)