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Science 26 March 1982:
Vol. 215. no. 4540, pp. 1637 - 1639
DOI: 10.1126/science.6122269
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Articles

Science, Vol 215, Issue 4540, 1637-1639
Copyright © 1982 by American Association for the Advancement of Science

articles

Inhibition of idiotype--anti-idiotype interaction for detection of a parasite antigen: a new immunoassay

P Potocnjak, F Zavala, R Nussenzweig, and V Nussenzweig

Described in this report is an immunoradiometric assay of general applicability that is based on a new principle: the inhibition of the interaction between monoclonal antibodies by an antigen. The advantages of this assay are that it measures concentrations of single epitopes, purified antigen is not required, and the reagents can be obtained in unlimited amounts and are homogeneous. Its features are particularly attractive when the antigen has not been purified and is a minor component of a complex mixture of molecule.




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Science. ISSN 0036-8075 (print), 1095-9203 (online)