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Science 5 March 1982:
Vol. 215. no. 4537, pp. 1255 - 1257
DOI: 10.1126/science.7058344
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Articles

Science, Vol 215, Issue 4537, 1255-1257
Copyright © 1982 by American Association for the Advancement of Science

articles

Hydrolysis of nerve gas by squid-type diisopropyl phosphorofluoridate hydrolyzing enzyme on agarose resin

FC Hoskin and AH Roush

An enzyme purified from squid nerve that hydrolyzes the cholinesterase inhibitor diisopropyl phosphorofluoridate (DFP) has now been coupled to agarose beads. A column of this agarose-DFPase hydrolyzes the nerve gas 1,2,2-trimethylpropyl methylphosphonofluoridate (Soman). Although the more inhibitory of the four diastereoisomers of Soman are hydrolyzed least rapidly, a column of sufficient length will accomplish 95 percent hydrolysis whether measured by fluoride release or loss of cholinesterase-inhibiting power. The results suggest a means for detoxifying unwanted chemical warfare agents.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Long Circulating Liposomes Encapsulating Organophosphorus Acid Anhydrolase in Diisopropylfluorophosphate Antagonism.
I. Petrikovics, T.-C. Cheng, D. Papahadjopoulos, K. Hong, R. Yin, J. J. DeFrank, J. Jaing, Z. H. Song, W. D. McGuinn, D. Sylvester, et al. (2000)
Toxicol. Sci. 57, 16-21
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