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Science 26 February 1982:
Vol. 215. no. 4536, pp. 1109 - 1112
DOI: 10.1126/science.6977845
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Articles

Science, Vol 215, Issue 4536, 1109-1112
Copyright © 1982 by American Association for the Advancement of Science

articles

Tonicity effects on intact single muscle fibers: relation between force and cell volume

J Gulati and A Babu

Contraction of isolated, intact frog muscle fibers under increasing tonicity of the external solution was studied by adding (i) effectively impermeant sodium chloride and sucrose and (ii) permeant potassium chloride. Force of isometric contraction decreased as a function of tonicity, independent of the permeability of the solute. In contrast, cell volume changed with tonicity in impermeant solutes and was constant with potassium chloride. The results are evidence that ionic strength in the sarcoplasm directly influences the contraction mechanism. Also, the findings show that force development is unaffected by changes in fiber volume, suggesting that the force per cross-bridge is constant at different distances between the thin and myofilaments. Finally, in light of the length-force relation, the results support the idea that cross-bridges are independent force generators.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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The influence of intracellular lactate and H+ on cell volume in amphibian skeletal muscle.
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Riding the Tides: K+ Concentration and Volume Regulation by Muscle Na+-K+-2Cl- Cotransport Activity.
A. R. Gosmanov, M. I. Lindinger, and D. B. Thomason (2003)
Physiology 18, 196-200
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The Filament Lattice of Striated Muscle.
B. M. MILLMAN (1998)
Physiol Rev 78, 359-391
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