Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 19 February 1982:
Vol. 215. no. 4535, pp. 985 - 987
DOI: 10.1126/science.7156978
Prev | Table of Contents | Next

Articles

Science, Vol 215, Issue 4535, 985-987
Copyright © 1982 by American Association for the Advancement of Science

articles

alpha A-crystallin messenger RNA of the mouse lens: more noncoding than coding sequences

CR King, T Shinohara, and J Piatigorsky

The 14S messenger RNA (1300 to 1500 nucleotides) for the alpha A chain of alpha-crystallin of the mammalian lens is nearly three times larger than required to code for the polypeptide that contains 173 amino acids. As a means of accounting for this anomaly, a complementary DNA clone for the mouse alpha A-crystallin messenger RNA was constructed in pBR322 and sequenced. Derivation of the protein sequence from the nucleic acid sequence showed that mouse alpha A-crystallin is similar to that of other organisms. The messenger RNA contains 536 nucleotides located on the 3' side of the coding region, excluding the polyadenylate stretch. This 3' sequence does not encode any other crystallin and has multiple termination codons in the three possible reading frames.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The mouse eye lens obsolescence (Elo) mutant: studies on crystallin gene expression and linkage analysis between the mutant locus and the gamma-crystallin genes..
P Quinlan, S Oda, M L Breitman, and L C Tsui (1987)
Genes & Dev. 1, 637-644
   Abstract »    PDF »
Deletion and duplication of DNA sequences is associated with the embryonic lethal phenotype of the t9 complementation group of the mouse t complex..
M Bucan, B G Herrmann, A M Frischauf, V L Bautch, V Bode, L M Silver, G R Martin, and H Lehrach (1987)
Genes & Dev. 1, 376-385
   Abstract »    PDF »
Selective loss of a family of gene transcripts in a hereditary murine cataract.
A. Garber, C Winkler, T Shinohara, C. King, G Inana, J Piatigorsky, and R. Gold (1985)
Science 227, 74-77
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)