Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 5 February 1982:
Vol. 215. no. 4533, pp. 677 - 679
DOI: 10.1126/science.7058331
Prev | Table of Contents | Next

Articles

Science, Vol 215, Issue 4533, 677-679
Copyright © 1982 by American Association for the Advancement of Science

articles

Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer

RS Goodenow, M McMillan, A Orn, M Nicolson, N Davidson, JA Frelinger, and L Hood

Gene transfer and immunoselection were used in the identification of a BALB/c genomic clone containing an H-2Ld gene (clone 27.5). Transformation of thymidine kinase-negative C3H mouse L cells with the cloned 27.5 DNA together with the herpes simplex virus tk gene produced transformants expressing Ld molecules detected by radioimmune assay with monoclonal hybridoma antibodies to Ld antigens. The foreign Ld gene products expressed by cloned mouse L cell transformants were shown to be virtually indistinguishable from BALB/c spleen Ld molecules by two-dimensional electrophoretic analysis of H-2Ld immunoprecipitates. These results indicate that the genomic clone 27.5 contains a functional BALB/c H-2Ld gene and demonstrate the usefulness of this approach for identifying the gene products encoded by cloned genes which are members of a multigene family. Furthermore, the ability to place cell-surface recognition molecules on the surfaces of foreign cells provides a powerful opportunity for functional analyses of these molecules.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Histocompatibility antigens on murine tumors.
R. Goodenow, J. Vogel, and R. Linsk (1985)
Science 230, 777-783
   Abstract »    PDF »
Expression of a microinjected porcine class I major histocompatibility complex gene in transgenic mice.
W. Frels, J. Bluestone, R. Hodes, M. Capecchi, and D. Singer (1985)
Science 228, 577-580
   Abstract »    PDF »
Detection of antibodies to herpes simplex virus with a continuous cell line expressing cloned glycoprotein D.
P. Berman, D Dowbenko, L. Lasky, and C. Simonsen (1983)
Science 222, 524-527
   Abstract »    PDF »
Genes of the Major Histocompatibility Complex.
L. Hood, M. Steinmetz, R. Goodenow, K. Eakle, D. Fisher, J. Kobori, B. Malissen, M. Malissen, M. McMillan, J. McNicholas, et al. (1983)
Cold Spring Harb Symp Quant Biol 47, 1051-1065
   Abstract »    PDF »
DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.
K. Moore, B. Sher, Y. Sun, K. Eakle, and L Hood (1982)
Science 215, 679-682
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)