Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 8 January 1982:
Vol. 215. no. 4529, pp. 166 - 168
DOI: 10.1126/science.7053567
Prev | Table of Contents | Next

Articles

Science, Vol 215, Issue 4529, 166-168
Copyright © 1982 by American Association for the Advancement of Science

articles

Liposomes as gene carriers: efficient transformation of mouse L cells by thymidine kinase gene

M Schaefer-Ridder, Y Wang, and PH Hofschneider

Stable transformation of mouse L cells deficient in thymidine kinase was achieved by liposome-mediated transfer of a recombinant plasmid carrying the thymidine kinase gene. Ten percent of the recipient cells expressed thymidine kinase activity. The transformed phenotype (for example, 200 out of 10(6) cells) was stable under selective and nonselective conditions. The liposome technique is compared with other methods currently used for gene transfer.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Gene transfer by polyoma-like particles assembled in a cell-free system.
S. Slilaty and H. Aposhian (1983)
Science 220, 725-727
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)