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Science 1 January 1982:
Vol. 215. no. 4528, pp. 64 - 66
DOI: 10.1126/science.7053559
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Articles

Science, Vol 215, Issue 4528, 64-66
Copyright © 1982 by American Association for the Advancement of Science

articles

Phagocytosis: flow cytometric quantitation with fluorescent microspheres

JA Steinkamp, JS Wilson, GC Saunders, and CC Stewart

The phagocytosis of uniform fluorescent latex particles by pulmonary macrophages in the rat was analyzed by flow cytometric methods. The percentage of phagocytic macrophages and the number of particles per cell were determined from cell-size and fluorescence histograms. A comparison of in vivo and in vitro phagocytosis data showed that the percentage of phagocytic lavaged macrophages reflected the availability of instilled particles. With sodium azide used to model phagocytosis inhibition, it was shown that the percentage of phagocytic cells and the number of particles per cell can be determined simultaneously.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)