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Science 4 December 1981:
Vol. 214. no. 4525, pp. 1129 - 1131
DOI: 10.1126/science.7302583
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Articles

Science, Vol 214, Issue 4525, 1129-1131
Copyright © 1981 by American Association for the Advancement of Science

articles

Regulation of leucine metabolism in man: a stable isotope study

DE Matthews, DM Bier, MJ Rennie, RH Edwards, D Halliday, DJ Millward, and GA Clugston

Leucine catabolism is regulated by either of the first two degradative steps: (reversible) transamination to the keto acid or subsequent decarboxylation. A method is described to measure rates of leucine transamination, reamination, and keto acid oxidation. The method is applied directly to humans by infusing the nonradioactive tracer, L-[15N,1-13C]leucine. Leucine transamination was found to be operating several times faster than the keto acid decarboxylation and to be of equal magnitude in adult human males under two different dietary conditions, postabsorptive and fed. These results indicate that decarboxylation, not transamination, is the rate-limiting step in normal human leucine metabolism.


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