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Science 13 November 1981:
Vol. 214. no. 4522, pp. 801 - 803
DOI: 10.1126/science.6117129
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Articles

Science, Vol 214, Issue 4522, 801-803
Copyright © 1981 by American Association for the Advancement of Science

articles

Islets of Langerhans: dye coupling among immunocytochemically distinct cell types

RL Michaels and JD Sheridan

Cells in isolated rat islets of Langerhans were microinjected with Lucifer Yellow CH in a medium containing 16.7 millimolar glucose. Dye was rapidly transferred from the injected cell to neighboring islet cells without specificity with regard to the immunocytochemical identity of either the donor or the recipient cells. The transfer of dye between the islet cells (types A, B, and D) demonstrates homologous and heterologous cell coupling in a system where the normal proportions and relationships of the cell types are maintained.


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Synchronization and entrainment of cytoplasmic Ca2+ oscillations in cell clusters prepared from single or multiple mouse pancreatic islets.
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On-line analysis of gap junctions reveals more efficient electrical than dye coupling between islet cells.
I. Quesada, E. Fuentes, E. Andreu, P. Meda, A. Nadal, and B. Soria (2003)
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Homologous and heterologous asynchronicity between identified {alpha}-, {beta}- and {delta}-cells within intact islets of Langerhans in the mouse.
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