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Science 13 November 1981:
Vol. 214. no. 4522, pp. 801 - 803
DOI: 10.1126/science.6117129
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Articles

Science, Vol 214, Issue 4522, 801-803
Copyright © 1981 by American Association for the Advancement of Science

articles

Islets of Langerhans: dye coupling among immunocytochemically distinct cell types

RL Michaels and JD Sheridan

Cells in isolated rat islets of Langerhans were microinjected with Lucifer Yellow CH in a medium containing 16.7 millimolar glucose. Dye was rapidly transferred from the injected cell to neighboring islet cells without specificity with regard to the immunocytochemical identity of either the donor or the recipient cells. The transfer of dye between the islet cells (types A, B, and D) demonstrates homologous and heterologous cell coupling in a system where the normal proportions and relationships of the cell types are maintained.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell coupling in mouse pancreatic {beta}-cells measured in intact islets of Langerhans.
Q. Zhang, J. Galvanovskis, F. Abdulkader, C. J Partridge, S. O Gopel, L. Eliasson, and P. Rorsman (2008)
Phil Trans R Soc A 366, 3503-3523
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Cx36-Mediated Coupling Reduces {beta}-Cell Heterogeneity, Confines the Stimulating Glucose Concentration Range, and Affects Insulin Release Kinetics.
S. Speier, A. Gjinovci, A. Charollais, P. Meda, and M. Rupnik (2007)
Diabetes 56, 1078-1086
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Glucose Induces Glucagon Release Pulses Antisynchronous with Insulin and Sensitive to Purinoceptor Inhibition.
E. Grapengiesser, A. Salehi, S. S. Qader, and B. Hellman (2006)
Endocrinology 147, 3472-3477
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Synchronization and entrainment of cytoplasmic Ca2+ oscillations in cell clusters prepared from single or multiple mouse pancreatic islets.
M. Zarkovic and J.-C. Henquin (2004)
Am J Physiol Endocrinol Metab 287, E340-E347
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Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic {beta}-cells.
D. Caton, A. Calabrese, C. Mas, V. Serre-Beinier, A. Charollais, D. Caille, R. Zufferey, D. Trono, and P. Meda (2003)
J. Cell Sci. 116, 2285-2294
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A reappraisal of the blood glucose homeostat which comprehensively explains the type 2 diabetes mellitus-syndrome X complex.
J. H Koeslag, P. T Saunders, and E. Terblanche (2003)
J. Physiol. 549, 333-346
   Abstract »    Full Text »    PDF »
On-line analysis of gap junctions reveals more efficient electrical than dye coupling between islet cells.
I. Quesada, E. Fuentes, E. Andreu, P. Meda, A. Nadal, and B. Soria (2003)
Am J Physiol Endocrinol Metab 284, E980-E987
   Abstract »    Full Text »    PDF »
Homologous and heterologous asynchronicity between identified {alpha}-, {beta}- and {delta}-cells within intact islets of Langerhans in the mouse.
A. Nadal, I. Quesada, and B. Soria (1999)
J. Physiol. 517, 85-93
   Abstract »    Full Text »    PDF »


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