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Science 16 October 1981:
Vol. 214. no. 4518, pp. 280 - 287
DOI: 10.1126/science.7280693
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Articles

Science, Vol 214, Issue 4518, 280-287
Copyright © 1981 by American Association for the Advancement of Science

articles

Tandem mass spectrometry

FW McLafferty

Coupling mass spectrometers in series provides a new technique that has many advantages for the analysis of specific organic compounds in complex mixtures. Sensitivity to picograms of targeted compounds can be achieved with high specificity and nearly instantaneous response. The targeted compound is selectively ionized, and its characteristic ions are separated from most others of the mixture in the first mass spectrometer. The selected primary ions are then decomposed by collision, and from the resulting products the final mass analyzer selects secondary ions characteristic of the targeted compound. Tandem mass spectrometry can achieve specificities and sensitivities equivalent of those of methods such as radioimmunoassay and gas chromatography/mass spectrometry, while performing analyses in much shorter times.


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