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Science 18 September 1981:
Vol. 213. no. 4514, pp. 1385 - 1388
DOI: 10.1126/science.6115471
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Articles

Science, Vol 213, Issue 4514, 1385-1388
Copyright © 1981 by American Association for the Advancement of Science

articles

A conjugate of alpha-amanitin and monoclonal immunoglobulin G to Thy 1.2 antigen is selectively toxic to T lymphoma cells

MT Davis and JF Preston 3rd

A covalent conjugate of an alpha-amanitin azo derivative and a monoclonal immunoglobulin G to the Thy 1.2 antigen on murine T lymphocytes was synthesized. The conjugate was 375- to 750-fold more inhibitory to murine T lymphoma S49.1 cells than the unconjugated derivative. At 0.7 X 10(-7) to 1.5 X 10(-7) M and at 4 X 10(-7) M amanitin equivalents, the conjugate inhibited protein synthesis in S49.1 cells by 50 percent and 80 to 96 percent, respectively. At these concentrations, mutant Thy l-deficient S49 cells and other murine lymphoma lacking Thy l altogether or carrying Thy 1.1 antigens were unaffected. This result demonstrated the potential for targeting amanitin to specific cell types.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inhibition of human acute lymphoblastic leukemia cells by immunotoxins: potentiation by chloroquine.
S Ramakrishnan and L. Houston (1984)
Science 223, 58-61
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