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Science 14 August 1981:
Vol. 213. no. 4509, pp. 765 - 767
DOI: 10.1126/science.6454965
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Articles

Science, Vol 213, Issue 4509, 765-767
Copyright © 1981 by American Association for the Advancement of Science

articles

Fidelity of mammalian DNA polymerases

TA Kunkel and LA Loeb

The fidelity of copying natural DNA in vitro with each of the three classes of eukaryotic DNA polymerases has been determined. DNA polymerases-beta and -gamma are highly inaccurate, catalyzing noncomplementary single-base substitution at a frequency between 1/3000 and 1/8000. DNA polymerase-alpha is substantially more accurate, with an error rate of 1/30,000. When the error rates of these DNA polymerases are considered in the context of the accuracy of DNA replicative processes in vivo, it seems likely that other factors must exist in mammalian cells which are involved in the accurate replication and maintenance of the genetic information.


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Mitochondrial C-tract Alteration in Premalignant Lesions of the Head and Neck: A Marker for Progression and Clonal Proliferation.
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Mitochondrial DNA Somatic Mutations (Point Mutations and Large Deletions) and Mitochondrial DNA Variants in Human Thyroid Pathology : A Study with Emphasis on Hurthle Cell Tumors.
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Mutations in the Primer Grip Region of HIV Reverse Transcriptase Can Increase Replication Fidelity.
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The Role of 3'-5' Exonucleolytic Proofreading and Mismatch Repair in Yeast Mitochondrial DNA Error Avoidance.
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The Chromosome Bias of Misincorporations During Double-Strand Break Repair Is Not Altered in Mismatch Repair–Defective Strains of Saccharomyces cerevisiae.
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Replication of Template-Primers Containing Propanodeoxyguanosine by DNA Polymerase beta . INDUCTION OF BASE PAIR SUBSTITUTION AND FRAMESHIFT MUTATIONS BY TEMPLATE SLIPPAGE AND DEOXYNUCLEOSIDE TRIPHOSPHATE STABILIZATION.
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Incorporation of the Guanosine Triphosphate Analogs 8-Oxo-dGTP and 8-NH2-dGTP by Reverse Transcriptases and Mammalian DNA Polymerases.
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Rapid evolution of RNA genomes.
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A Single Highly Mutable Catalytic Site Amino Acid Is Critical for DNA Polymerase Fidelity.
P. H. Patel, H. Kawate, E. Adman, M. Ashbach, and L. A. Loeb (2001)
J. Biol. Chem. 276, 5044-5051
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The Interaction of DNA Mismatch Repair Proteins with Human Exonuclease I.
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