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Science 31 July 1981:
Vol. 213. no. 4507, pp. 549 - 551
DOI: 10.1126/science.213.4507.549

Articles

Nullisomic Tetrahymena: Eliminating Germinal Chromosomes

PETER J. BRUNS 1 and TRUDY E. B. BRUSSARD 1

1 Section of Genetics and Development, Cornell University, Ithaca, New York 14853

Germinal and somatic functions in Tetrahymena are performed separately by the micro- and macronuclei, respectively. Cells with haploid micronuclei were mated with diploids to yield monosomic progeny. These were induced to undergo a form of self-fertilization, generating cells lacking both copies of one or more of the five chromosomes in the micronucleus while still possessing a complete macronuclear genome.

Submitted on April 28, 1981


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tetrahymena Micronuclear Genome Mapping: A High-Resolution Meiotic Map of Chromosome 1L.
S. Wickert and E. Orias (2000)
Genetics 154, 1141-1153
   Abstract »    Full Text »
Tetrahymena Macronuclear Genome Mapping: Colinearity of Macronuclear Coassortment Groups and the Micronuclear Map on Chromosome 1L.
S. Wickert, L. Nangle, S. Shevel, and E. Orias (2000)
Genetics 154, 1155-1167
   Abstract »    Full Text »



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Science. ISSN 0036-8075 (print), 1095-9203 (online)