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Science 24 July 1981:
Vol. 213. no. 4506, pp. 457 - 459
DOI: 10.1126/science.6264603
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Articles

Science, Vol 213, Issue 4506, 457-459
Copyright © 1981 by American Association for the Advancement of Science

articles

Calcitonin messenger RNA encodes multiple polypeptides in a single precursor

JW Jacobs, RH Goodman, WW Chin, PC Dee, JF Habener, NH Bell, and JT Potts Jr

Recombinant DNA techniques were used to analyze the structure of the messenger RNA encoding a precursor of calcitonin, a small calcium-regulating hormone of 32 amino acids. Analyses of the nucleotide sequences of cloned complementary DNA's comprising the entire coding sequence of the messenger RNA revealed that calcitonin is flanked at both its amino and carboxyl termini by peptide extensions linked to the hormone by short sequences of basic amino acids. The location of glycine next to the carboxyl terminal prolinamide of calcitonin is consistent with indications that glycine is required for the enzymatic amidation of proline to the prolinamide. During cellular biosynthesis, calcitonin arises from a large precursor protein by cleavages at both amino and carboxyl terminal residues of the hormone. These findings raise questions concerning the regulation of these cleavages and the potential biological functions of the precursor extensions derived from these cleavages.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Ubiquitous Expression of the Calcitonin-I Gene in Multiple Tissues in Response to Sepsis.
B. Müller, J. C. White, E. S. Nylén, R. H. Snider, K. L. Becker, and J. F. Habener (2001)
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Alternative RNA processing: determining neuronal phenotype.
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Molecular Events in Developmental Regulation of Neuroendocrine Genes: Characterization of the Novel Neuropeptide CGRP.
R.M. Evans, S. Amara, and M.G. Rosenfeld (1983)
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