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Science 17 July 1981:
Vol. 213. no. 4505, pp. 344 - 346
DOI: 10.1126/science.213.4505.344
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Articles

Hemoglobin Kinetics of the Galápagos Rift Vent Tube Worm Riftia pachyptila Jones (Pogonophora; Vestimentifera)

JONATHAN B. WITTENBERG 1, ROGER J. MORRIS 2, QUENTIN H. GIBSON 2, and MEREDITH L. JONES 3

1 Department of Physiology, Albert Einstein College of Medicine, Bronx, Nw York 10461
2 Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853
3 Department of Invertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, D.C. 20560

Kinetics of the reactions of Riftia pachyptila hemoglobin with oxygen were followed spectrophotometrically by stopped-flow and laser flash photolysis techniques. The rate of oxygen dissociation increases eightfold over the range of 5° to 20°C (k = 2.2 sec–1at 10°C). Oxygen recombination after flash photolysis was biphasic. The rates of both slow and fast phases of the reaction were independent of temperature from 0° to 20°C(k'fast = 7 x 106; k'slow = 1 x 166 liter mole –1 sec–1). As the oxygen affinity is relatively temperature independent, analysis in terms of the two-state model of cooperativity requires that the conformational equilibrium constant L decrease by about 50-fold between 3°and 15°C.

Submitted on July 28, 1980
Revised on January 6, 1981


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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