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Science 10 July 1981:
Vol. 213. no. 4504, pp. 230 - 233
DOI: 10.1126/science.7244630
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Articles

Science, Vol 213, Issue 4504, 230-233
Copyright © 1981 by American Association for the Advancement of Science

articles

Freeze-fracture cytochemistry: replicas of critical point-dried cells and tissues after fracture-label

PP da Silva, B Kachar, MR Torrisi, C Brown, and C Parkison

Applications of the new fracture-labeling techniques for the observation of cytochemical labels on platinum-carbon replicas are described. Frozen cells, embedded in a cross-linked protein matrix, and frozen tissues are fractured with a scalpel under liquid nitrogen, thawed, labeled, dehydrated by the critical point drying method, and replicated. This method allows direct, high-resolution, two-dimensional chemical and immunological characterization of the cellular membranes in situ, as well as detection of sites within cross-fractured cytoplasm and extracellular matrix.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Co-localization of Dystrophin and ß-Dystroglycan Demonstrated in En Face View by Double Immunogold Labeling of Freeze-fractured Skeletal Muscle.
M. J. Cullen, J. Walsh, S. A. Stevenson, S. Rothery, and N. J. Severs (1998)
J. Histochem. Cytochem. 46, 945-954
   Abstract »    Full Text »
Spatial Relationship of the C-Terminal Domains of Dystrophin and ß-Dystroglycan in Cardiac Muscle Support a Direct Molecular Interaction at the Plasma Membrane Interface.
S. Stevenson, S. Rothery, M. J. Cullen, and N. J. Severs (1998)
Circ. Res. 82, 82-93
   Abstract »    Full Text »    PDF »
Freeze-fracture Cytochemistry: A New Method Combining Immunocytochemistry and Enzyme Cytochemistry on Replicas.
T. Takizawa, T. Saito, and J. M. Robinson (1998)
J. Histochem. Cytochem. 46, 11-18
   Abstract »    Full Text »
Dystrophin Is Not a Specific Component of the Cardiac Costamere.
S. Stevenson, S. Rothery, M. J. Cullen, and N. J. Severs (1997)
Circ. Res. 80, 269-280
   Abstract »    Full Text »


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