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Science 5 June 1981:
Vol. 212. no. 4499, pp. 1146 - 1148
DOI: 10.1126/science.7233207
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Articles

Science, Vol 212, Issue 4499, 1146-1148
Copyright © 1981 by American Association for the Advancement of Science

articles

Cultivation in vitro of the vivax-type malaria parasite Plasmodium cynomolgi

P Nguyen-Dinh, AL Gardner, CC Campbell, JC Skinner, and WE Collins

The vivax-type simian malaria parasite Plasmodium cynomologi was cultured in vitro by both the candle jar method and the continuous flow technique, with rhesus monkey erythrocytes and RPMI 1640 medium supplemented with Hepes buffer and human serum. After 6 weeks in culture, the growth of the parasite had permitted a 5 X 10(6) cumulative dilution of the original inoculum. Cultured parasites remained infective to rhesus monkeys and exhibited a reversible decrease in the ameboid behavior of their trophozoites.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cultivation of Plasmodium spp..
F. L. Schuster (2002)
Clin. Microbiol. Rev. 15, 355-364
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Continuous in vitro propagation of the malaria parasite Plasmodium vivax.
C. F. Golenda, J. Li, and R. Rosenberg (1997)
PNAS 94, 6786-6791
   Abstract »    Full Text »    PDF »


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