Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 March 1981:
Vol. 211. no. 4489, pp. 1452 - 1454
DOI: 10.1126/science.6970413
Prev | Table of Contents | Next

Articles

Science, Vol 211, Issue 4489, 1452-1454
Copyright © 1981 by American Association for the Advancement of Science

articles

An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes

T Maciag, RE Nemore, R Weinstein, and BA Gilchrest

Human keratinocytes, derived from the skin of newborns and of adults, were grown in the complete absence of serum, in a hormone-supplemented medium on fibronectin-coated cell culture dishes at low seed density. The cell culture medium consisted of Medium 199 containing epidermal growth factor, triiodothyronine, hydrocortisone, Cohn fraction IV, insulin, transferrin, bovine brain extract, and trace elements. Removal of the brain extract from the hormone supplement had a greater negative impact on proliferation of the keratinocyte cultures than did the removal of epidermal growth factor, hydrocortisone, and triiodothyronine or Cohn fraction IV. The growth of keratinocytes in this hormone-supplemented medium suggests that control of keratinocyte growth depends in large part on endocrine stimulation by other body organs, including the brain.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The use of human serum in supporting the in vitro and in vivo proliferation of human conjunctival epithelial cells.
L P K Ang, D T H Tan, C J Y Seah, and R W Beuerman (2005)
Br. J. Ophthalmol. 89, 748-752
   Abstract »    Full Text »    PDF »
beta-catenin signalling modulates proliferative potential of human epidermal keratinocytes independently of intercellular adhesion.
A. Zhu and F. Watt (1999)
Development 126, 2285-2298
   Abstract »    PDF »
Cell kinetic characterization of growth arrest in cultured human keratinocytes.
F van Ruissen, P. van Erp, G. de Jongh, J. Boezeman, P. van de Kerkhof, and J Schalkwijk (1994)
J. Cell Sci. 107, 2219-2228
   Abstract »    PDF »
Growth Factors: Their Biology and Promise in Dermatologic Diseases and Tissue Repair.
M. Rothe and V. Falanga (1989)
Arch Dermatol 125, 1390-1398
   Abstract »    PDF »
Control of Growth and Differentiation In Vitro of Human Keratinocytes Cultured in Serum-free Medium.
G. D. Shipley and M. R. Pittelkow (1987)
Arch Dermatol 123, 1541a-1544a
   Abstract »    PDF »
Heparin binds endothelial cell growth factor, the principal endothelial cell mitogen in bovine brain.
T Maciag, T Mehlman, R Friesel, and A. Schreiber (1984)
Science 225, 932-935
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)