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Science 27 February 1981:
Vol. 211. no. 4485, pp. 937 - 940
DOI: 10.1126/science.211.4485.937
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Articles

Azidoatrazine: Photoaffinity Label for the Site of Triazine Herbicide Action in Chloroplasts

GARY GARDNER 1

1 Shell Development Company, Biological Sciences Research Center, Modesto, California 95352

Binding of the 4-azido analog of the herbicide atrazine to pea chloroplast membranes was compared with that of atrazine. When [14C]azidoatrazine was treated with 300-nanometer ultraviolet light in situ, reversibility of binding was lost in proportion to the duration of irradiation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of chloroplast membranes irradiated in the presence of [l4C]-azidoatrazine indicated radioactivity in only one region, corresponding to a protein with a molecular weight of approximately 32,000. Azidoatrazine is a photoaffinity reagent for the triazine binding site in chloroplasts and serves as a label to identify this site, which may be the apoprotein of the secondary electron acceptor in photosystem II.

Submitted on August 4, 1980
Revised on September 29, 1980


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Molecular Basis of Herbicide Resistance in Amaranthus hybridus.
J. HIRSCHBERG and L. MCINTOSH (1983)
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Science. ISSN 0036-8075 (print), 1095-9203 (online)