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Science 13 February 1981:
Vol. 211. no. 4483, pp. 712 - 715
DOI: 10.1126/science.6779379
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Articles

Science, Vol 211, Issue 4483, 712-715
Copyright © 1981 by American Association for the Advancement of Science

articles

Gap junctional conductance is a simple and sensitive function of intracellular pH

DC Spray, AL Harris, and MV Bennett

The pH of the cytoplasm (pHt) measured with pH-sensitive microelectrodes in cleavage-stage blastomeres of amphibian (Ambystoma) and teleost (Fundulus) embryos is about 7.7. In electrotonically coupled cell pairs, junctional conductance is rapidly and reversibly reduced by acidification of the cytoplasm. The relation between junctional conductance and pHi is the same for increasing and decreasing pH and is independent of the rate of change over a wide range. The relation is well fitted by a Hill curve with K = 50 nM (pK = 7.3) and n = 4 to 5. The closure of gap junction channels at low pHi appears to be a cooperative process involving several charged sites. The absence of hysteresis and identity of effects for fast and slow pHi changes implies that protons act directly on the channel macromolecules and not through an intermediate in the cytoplasm.


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