Science, Vol 210, Issue 4476, 1360-1363
Copyright © 1980 by American Association for the Advancement of Science
Comparison of the nucleic acid sequence of anglerfish and mammalian insulin mRNA's from cloned cDNA's
PM Hobart,
LP Shen,
R Crawford,
RL Pictet,
and
WJ Rutter
Anglerfish (Lophius americanus) insulin complementary DNA was cloned in bacterial plasmids, and its sequence was determined. Fish insulin messenger RNA is larger (1.5 times) than the messenger RNA encoding mammalian (rat and human) insulin, in part because of a larger C peptide (an additional six amino acids or 18 nucleotides in length) but mainly because of increases in the 5' and 3' untranslated regions. Comparison of the fish, rat, and human insulin messenger RNA (from the complementary DNA) reveals that, in addition to the regions coding for the A and B peptides, sequence conservation is limited to a segment within the 5' untranslated region which may be involved in ribosomal binding, two small segments of the signal peptide, and two stretches of sequence in the 3' untranslated region.
