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Science 21 November 1980:
Vol. 210. no. 4472, pp. 912 - 914
DOI: 10.1126/science.7434005
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Articles

Science, Vol 210, Issue 4472, 912-914
Copyright © 1980 by American Association for the Advancement of Science

articles

Transporting renal epithelium: culture in hormonally defined serum-free medium

DM Jefferson, MH Cobb, JF Gennaro Jr, and WN Scott

A hormonally defined medium was used to isolate a homogeneous epithelioid cell population from canine kidney. Monolayers of these cells form domes, an indication of active ion transport, and this process is inhibited by ouabain. This technique allows the isolation of primary cultures of renal epithelial cells, free of fibroblasts, for the characterization of biochemical and physiological properties related to renal function.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Epithelial sodium channel in human epidermal keratinocytes: expression of its subunits and relation to sodium transport and differentiation.
M Brouard, M Casado, S Djelidi, Y Barrandon, and N Farman (1999)
J. Cell Sci. 112, 3343-3352
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