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Science 31 October 1980:
Vol. 210. no. 4469, pp. 537 - 539
DOI: 10.1126/science.7423202
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Articles

Science, Vol 210, Issue 4469, 537-539
Copyright © 1980 by American Association for the Advancement of Science

articles

Human monoclonal antibody against Forssman antigen

R Nowinski, C Berglund, J Lane, M Lostrom, I Bernstein, W Young, SI Hakomori, L Hill, and M Cooney

Hybrid cells formed between human lymphocytes and mouse myeloma cells produce human immunoglobulin in culture. Stable antibody-producing cell lines can be isolated after multiple cycles of low-density passage, cloning, and continued selection for immunoglobulin production. The origin and characteristics of a hybrid of human and mouse cells is described. This hybrid produces high concentrations (8.3 micrograms per milliliter) of human immunoglobulin M reactive with the terminal disaccharide of the Forssman glycolipid. These findings point to the potential use of human-mouse hybrid cells as a source of human monoclonal antibodies for therapeutic and diagnostic purposes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Activation and Regulation of Human Immune Responses: Implications in Normal and Disease States.
A. S. FAUCI, H. C. LANE, and D. J. VOLKMAN (1983)
Ann Intern Med 99, 61-75
   Abstract »    PDF »
Bovine x mouse hybridomas that secrete bovine immunoglobulin G1.
S Srikumaran, A. Guidry, and R. Goldsby (1983)
Science 220, 522-524
   Abstract »    PDF »
Monoclonal Antibodies: Clinical Relevance to Pediatrics.
R. A. Insel and F. Gigliotti (1983)
Arch Pediatr Adolesc Med 137, 69-76
   Abstract »    PDF »


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