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Science 10 October 1980:
Vol. 210. no. 4466, pp. 205 - 207
DOI: 10.1126/science.6251552
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Articles

Science, Vol 210, Issue 4466, 205-207
Copyright © 1980 by American Association for the Advancement of Science

articles

Asymmetry of the acetylcholine channel revealed by quaternary anesthetics

R Horn, MS Brodwick, and WD Dickey

Tissue-cultured rat myoballs were examined electrophysiologically with a suction pipette, which was used for voltage clamping and internal perfusion. The lidocaine derivative QX-314 caused a time- and membrane potentia-dependent block of acetylcholine-induced current only when applied from the extracellular membrane surface. The same compounds caused a use-dependent block of the sodium channel only from the intracellular membrane surface. These experiments demonstrate a fundamental asymmetry of the acetylcholine receptor-channel complex.


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Ethanol Potentiation of GABAergic Synaptic Transmission May Be Self-Limiting: Role of Presynaptic GABAB Receptors.
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Enhanced Inhibition of a Mutant Neuronal Nicotinic Acetylcholine Receptor by Agonists: Protection of Function by (E)-N-Methyl-4-(3-pyridinyl)-3-butene-1-amine (TC-2403).
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Local Anesthetics Noncompetitively Inhibit Function of Four Distinct Nicotinic Acetylcholine Receptor Subtypes.
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Inhibition of Wild-Type and Mutant Neuronal Nicotinic Acetylcholine Receptors by Local Anesthetics.
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Delimiting the Binding Site for Quaternary Ammonium Lidocaine Derivatives in the Acetylcholine Receptor Channel.
J. M. Pascual and A. Karlin (1998)
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Inaugural Article: Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method.
G. G. Wilson and A. Karlin (2001)
PNAS 98, 1241-1248
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