Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 10 October 1980:
Vol. 210. no. 4466, pp. 205 - 207
DOI: 10.1126/science.6251552
Prev | Table of Contents | Next

Articles

Science, Vol 210, Issue 4466, 205-207
Copyright © 1980 by American Association for the Advancement of Science

articles

Asymmetry of the acetylcholine channel revealed by quaternary anesthetics

R Horn, MS Brodwick, and WD Dickey

Tissue-cultured rat myoballs were examined electrophysiologically with a suction pipette, which was used for voltage clamping and internal perfusion. The lidocaine derivative QX-314 caused a time- and membrane potentia-dependent block of acetylcholine-induced current only when applied from the extracellular membrane surface. The same compounds caused a use-dependent block of the sodium channel only from the intracellular membrane surface. These experiments demonstrate a fundamental asymmetry of the acetylcholine receptor-channel complex.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Distinct Mechanisms of Ethanol Potentiation of Local and Paracapsular GABAergic Synapses in the Rat Basolateral Amygdala.
Y. Silberman, L. Shi, J. K. Brunso-Bechtold, and J. L. Weiner (2008)
J. Pharmacol. Exp. Ther. 324, 251-260
   Abstract »    Full Text »    PDF »
Ethanol Potentiation of GABAergic Synaptic Transmission May Be Self-Limiting: Role of Presynaptic GABAB Receptors.
O. J. Ariwodola and J. L. Weiner (2004)
J. Neurosci. 24, 10679-10686
   Abstract »    Full Text »    PDF »
Enhanced Inhibition of a Mutant Neuronal Nicotinic Acetylcholine Receptor by Agonists: Protection of Function by (E)-N-Methyl-4-(3-pyridinyl)-3-butene-1-amine (TC-2403).
R. L. Papke (2002)
J. Pharmacol. Exp. Ther. 301, 765-773
   Abstract »    Full Text »    PDF »
Local Anesthetics Noncompetitively Inhibit Function of Four Distinct Nicotinic Acetylcholine Receptor Subtypes.
C. L. Gentry and R. J. Lukas (2001)
J. Pharmacol. Exp. Ther. 299, 1038-1048
   Abstract »    Full Text »    PDF »
Inhibition of Wild-Type and Mutant Neuronal Nicotinic Acetylcholine Receptors by Local Anesthetics.
R. L. Papke, B. A. Horenstein, and A. N. Placzek (2001)
Mol. Pharmacol. 60, 1365-1374
   Abstract »    Full Text »    PDF »
Inaugural Article: Acetylcholine receptor channel structure in the resting, open, and desensitized states probed with the substituted-cysteine-accessibility method.
G. G. Wilson and A. Karlin (2001)
PNAS 98, 1241-1248
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)