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Science 26 September 1980:
Vol. 209. no. 4464, pp. 1538 - 1541
DOI: 10.1126/science.6159680
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Articles

Science, Vol 209, Issue 4464, 1538-1541
Copyright © 1980 by American Association for the Advancement of Science

articles

Cell lineage analysis by intracellular injection of fluorescent tracers

DA Weisblat, SL Zackson, SS Blair, and JD Young

Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Helobdella (Leech): A Model for Developmental Studies.
D. A. Weisblat and D.-H. Kuo (2009)
CSH Protocols 2009, pdb.emo121
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Applications of mRNA injections for analyzing cell lineage and asymmetric cell divisions during segmentation in the leech Helobdella robusta.
S. O. Zhang and D. A. Weisblat (2005)
Development 132, 2103-2113
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Cell interactions that affect axonogenesis in the leech Theromyzon rude.
D. H. Shain, D. K. Stuart, F. Z. Huang, and D. A. Weisblat (2004)
Development 131, 4143-4153
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A hedgehog homolog regulates gut formation in leech (Helobdella).
D. Kang, F. Huang, D. Li, M. Shankland, W. Gaffield, and D. A. Weisblat (2003)
Development 130, 1645-1657
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Segmentation of the central nervous system in leech.
D. Shain, D. Stuart, F. Huang, and D. Weisblat (2000)
Development 127, 735-744
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Magnetic resonance microscopy of embryonic cell lineages and movements.
R. Jacobs and S. Fraser (1994)
Science 263, 681-684
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Segment and cell type lineage restrictions during pharyngeal arch development in the zebrafish embryo.
T. Schilling and C. Kimmel (1994)
Development 120, 483-494
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Cell lineage analysis of the expression of an engrailed homolog in leech embryos.
D Lans, C. Wedeen, and D. Weisblat (1993)
Development 117, 857-871
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Tissue-Specific Cell Lineages Originate in the Gastrula of the Zebrafish.
C. B. KIMMEL and R. M. WARGA (1986)
Science 231, 365-368
   Abstract »    PDF »
The Molecular Organization of the Leech Nervous System.
R.D. McKay, S. Hockfield, J. Johansen, and K. Frederiksen (1983)
Cold Spring Harb Symp Quant Biol 48, 599-610
   Abstract »    PDF »
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate.
H Giloh and J. Sedat (1982)
Science 217, 1252-1255
   Abstract »    PDF »
Serotonin analog selectively ablates indentified neurons in the leech embryo.
J. Glover and A. Kramer (1982)
Science 216, 317-319
   Abstract »    PDF »


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