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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1422 - 1427
DOI: 10.1126/science.6251549
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Articles

Science, Vol 209, Issue 4463, 1422-1427
Copyright © 1980 by American Association for the Advancement of Science

articles

Expression of a bacterial gene in mammalian cells

RC Mulligan and P Berg

Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.


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