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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1406 - 1414
DOI: 10.1126/science.6251548
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Articles

Science, Vol 209, Issue 4463, 1406-1414
Copyright © 1980 by American Association for the Advancement of Science

articles

Promoter sequences of eukaryotic protein-coding genes

J Corden, B Wasylyk, A Buchwalder, P Sassone-Corsi, C Kedinger, and P Chambon

In vitro genetic techniques were used to study the sequence requirements for the initiation of specific transcription. Deletion mutants were constructed around the putative promoter of the adenovirus-2 major late and chicken conalbumin genes. Specific transcription in vitro by RNA polymerase B together with a HeLa cell cytoplasmic extract was used as the test for promoter function. With this approach sequences which are essential for the initiation of specific transcription in vitro, were shown to be located between 12 and 32 base pairs upstream from the 5' end of these genes.


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Structure and Expression of the Murine Muscle Adenylosuccinate Synthetase Gene.
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RNA polymerase II initiation factor interactions and transcription start site selection.
Y Li, P. Flanagan, H Tschochner, and R. Kornberg (1994)
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DNA melting on yeast RNA polymerase II promoters.
C Giardina and J. Lis (1993)
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A G Bang and J W Posakony (1992)
Genes & Dev. 6, 1752-1769
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Retrovirus activation in embryonal carcinoma cells by cellular promoters..
I Peckham, S Sobel, J Comer, R Jaenisch, and E Barklis (1989)
Genes & Dev. 3, 2062-2071
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The Drosophila E74 promoter contains essential sequences downstream from the start site of transcription..
C S Thummel (1989)
Genes & Dev. 3, 782-792
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Physical analysis of transcription preinitiation complex assembly on a class II gene promoter.
M. Van Dyke, R. Roeder, and M Sawadogo (1988)
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Expression of an activated ras gene causes developmental abnormalities in transgenic Drosophila melanogaster..
J G Bishop and V G Corces (1988)
Genes & Dev. 2, 567-577
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Constraints on spacing between transcription factor binding sites in a simple adenovirus promoter..
L Wu and A Berk (1988)
Genes & Dev. 2, 403-411
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A deletion truncating the gonadotropin-releasing hormone gene is responsible for hypogonadism in the hpg mouse.
A. Mason, J. Hayflick, R. Zoeller, W. Young 3rd, H. Phillips, K Nikolics, and P. Seeburg (1986)
Science 234, 1366-1371
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Herpes simplex virus type-1 glycoprotein D gene: nucleotide sequence and expression in Escherichia coli.
R. Watson, J. Weis, J. Salstrom, and L. Enquist (1982)
Science 218, 381-384
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Transcriptional control signals of a eukaryotic protein-coding gene.
S. McKnight and R Kingsbury (1982)
Science 217, 316-324
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Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes.
P Rotwein, R Chyn, J Chirgwin, B Cordell, H. Goodman, and M. Permut (1981)
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Structure and in vitro transcription of human globin genes.
N. Proudfoot, M. Shander, J. Manley, M. Gefter, and T Maniatis (1980)
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J. Biol. Chem. 275, 27117-27122
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