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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1396 - 1400
DOI: 10.1126/science.6997991
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Articles

Science, Vol 209, Issue 4463, 1396-1400
Copyright © 1980 by American Association for the Advancement of Science

articles

Directed deletion of a yeast transfer RNA intervening sequence

RB Wallace, PF Johnson, S Tanaka, M Schold, K Itakura, and J Abelson

Many eukaryotic genes contain intevening sequences, segments of DNA that interrupt the continuity of the gene. They are removed from RNA transcripts of the gene by a process known as splicing. The intervening sequence in a yeast tyrosine transfer RNA (tRNA Tyr) suppressor gene was deleted in order to test its role in the expression of the gene. The altered gene and its parent were introduced into yeast by transformation. Both genes exhibited suppressor function, showing that the intervening sequence is not absolutely essential for the expression of this gene.


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