Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 19 September 1980:
Vol. 209. no. 4463, pp. 1392 - 1396
DOI: 10.1126/science.6251547
Prev | Table of Contents | Next

Articles

Science, Vol 209, Issue 4463, 1392-1396
Copyright © 1980 by American Association for the Advancement of Science

articles

Isolation of mutants of an animal virus in bacteria

KW Peden, JM Pipas, S Pearson-White, and D Nathans

Mutants of animal viruses can be isolated in bacteria by recombinant DNA methods. Since no viral functions are required for propagation of recombinants in bacteria, viral mutants with lethal changes in cis- or trans-acting elements can be isolated, as well as partially or conditionally defective mutants. In the cases of viruses with small DNA genomes, such as the tumorigenic simian virus 40 (SV40), the entire viral DNA can be inserted into the bacterial plasmid pBR322 and cloned in Escherichia coli. Recombinant plasmids with a single copy of SV40 DNA cause morphological transformation of mouse cells in culture with the same efficiency as SV40 DNA isolated from virus-infected monkey cells, but the recombinant DNA is noninfectious and replicates poorly in permissive cells. However, SV40 DNA excised from the plasmid replicates as well as authentic viral DNA and is fully infectious. SV40 mutants with small deletions or base substitutions have been isolated by in vitro site-specific or random local mutagenesis of recombinant DNA followed by cloning in E. coli. Many of the mutants thus isolated are defective in specific viral functions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification and functional analysis of the origins of DNA replication in the Cydia pomonella granulovirus genome.
S. Hilton and D. Winstanley (2007)
J. Gen. Virol. 88, 1496-1504
   Abstract »    Full Text »    PDF »
Inhibition of DNA replication of human papillomavirus by artificial zinc finger proteins..
T. Mino, T. Hatono, N. Matsumoto, T. Mori, Y. Mineta, Y. Aoyama, and T. Sera (2006)
J. Virol. 80, 5405-5412
   Abstract »    Full Text »    PDF »
Complete Nucleotide Sequence of Polyomavirus SA12.
P. Cantalupo, A. Doering, C. S. Sullivan, A. Pal, K. W. C. Peden, A. M. Lewis, and J. M. Pipas (2005)
J. Virol. 79, 13094-13104
   Abstract »    Full Text »    PDF »
Characterization of a Baculovirus Lacking the Alkaline Nuclease Gene.
K. Okano, A. L. Vanarsdall, and G. F. Rohrmann (2004)
J. Virol. 78, 10650-10656
   Abstract »    Full Text »    PDF »
Site-specific mutagenesis by triple helix-forming oligonucleotides containing a reactive nucleoside analog.
F. Nagatsugi, S. Sasaki, P. S. Miller, and M. M. Seidman (2003)
Nucleic Acids Res. 31, e31
   Abstract »    Full Text »    PDF »
Stimulation of DNA Replication from the Polyomavirus Origin by PCAF and GCN5 Acetyltransferases: Acetylation of Large T Antigen.
A.-Y. Xie, V. P. Bermudez, and W. R. Folk (2002)
Mol. Cell. Biol. 22, 7907-7918
   Abstract »    Full Text »    PDF »
Inhibition of Polyomavirus ori-Dependent DNA Replication by mSin3B.
A.-Y. Xie and W. R. Folk (2002)
J. Virol. 76, 11809-11818
   Abstract »    Full Text »    PDF »
DNA Replication Efficiency Depends on Transcription Factor-Binding Sites.
W. J. Turner and M. E. Woodworth (2001)
J. Virol. 75, 5638-5645
   Abstract »    Full Text »
Species-Specific Elements in the Large T-Antigen J Domain Are Required for Cellular Transformation and DNA Replication by Simian Virus 40.
C. S. Sullivan, J. D. Tremblay, S. W. Fewell, J. A. Lewis, J. L. Brodsky, and J. M. Pipas (2000)
Mol. Cell. Biol. 20, 5749-5757
   Abstract »    Full Text »
Effects of Exonuclease Activity and Nucleotide Selectivity of the Herpes Simplex Virus DNA Polymerase on the Fidelity of DNA Replication In Vivo.
Y. T. Hwang, B.-Y. Liu, C.-Y. Hong, E. J. Shillitoe, and C. B. C. Hwang (1999)
J. Virol. 73, 5326-5332
   Abstract »    Full Text »
Characterization of a Baculovirus-Encoded ATP-Dependent DNA Ligase.
M. N. Pearson and G. F. Rohrmann (1998)
J. Virol. 72, 9142-9149
   Abstract »    Full Text »    PDF »
Transactivation by the E2 Protein of Oncogenic Human Papillomavirus Type 31 Is Not Essential for Early and Late Viral Functions.
F. Stubenrauch, A. M. E. Colbert, and L. A. Laimins (1998)
J. Virol. 72, 8115-8123
   Abstract »    Full Text »    PDF »
Differential Requirements for Conserved E2 Binding Sites in the Life Cycle of Oncogenic Human Papillomavirus Type 31.
F. Stubenrauch, H. B. Lim, and L. A. Laimins (1998)
J. Virol. 72, 1071-1077
   Abstract »    Full Text »    PDF »
T Antigens Encoded by Replication-defective Simian Virus 40 Mutants dl1135 and 5080.
B. S. Collins and J. M. Pipas (1995)
J. Biol. Chem. 270, 15377-15384
   Abstract »    Full Text »    PDF »
The efficiency and timing of plasmid DNA replication in Xenopus eggs: correlations to the extent of prior chromatin assembly.
J. A. Sanchez, D. Marek, and L. J. Wangh (1992)
J. Cell Sci. 103, 907-918
   Abstract »    PDF »
The Autographa californica baculovirus genome: evidence for multiple replication origins.
M Pearson, R Bjornson, G Pearson, and G Rohrmann (1992)
Science 257, 1382-1384
   Abstract »    PDF »
Perturbed development of the mouse lens by polyomavirus large T antigen does not lead to tumor formation..
A E Griep, T Kuwabara, E J Lee, and H Westphal (1989)
Genes & Dev. 3, 1075-1085
   Abstract »    PDF »
Genetic engineering of novel genomes of large DNA viruses.
B Roizman and F. Jenkins (1985)
Science 229, 1208-1214
   Abstract »    PDF »
RNA splice site selection: evidence for a 5' leads to 3' scanning model.
K. Lang and R. Spritz (1983)
Science 220, 1351-1355
   Abstract »    PDF »
Multiple point mutations affecting the simian virus 40 enhancer.
H Weiher, M Konig, and P Gruss (1983)
Science 219, 626-631
   Abstract »    PDF »
Stability of DNA Triplexes on Shuttle Vector Plasmids in the Replication Pool in Mammalian Cells.
F.-L. M. Lin, A. Majumdar, L. C. Klotz, A. P. Reszka, S. Neidle, and M. M. Seidman (2000)
J. Biol. Chem. 275, 39117-39124
   Abstract »    Full Text »    PDF »
Targeted Gene Knockout by 2'-O-Aminoethyl Modified Triplex Forming Oligonucleotides.
N. Puri, A. Majumdar, B. Cuenoud, F. Natt, P. Martin, A. Boyd, P. S. Miller, and M. M. Seidman (2001)
J. Biol. Chem. 276, 28991-28998
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)