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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1385 - 1391
DOI: 10.1126/science.6251546
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Articles

Science, Vol 209, Issue 4463, 1385-1391
Copyright © 1980 by American Association for the Advancement of Science

articles

Tumor DNA structure in plant cells transformed by A. tumefaciens

P Zambryski, M Holsters, K Kruger, A Depicker, J Schell, M Van Montagu, and HM Goodman

Crown gall tumors are induced in plants by infection with the soil bacterium Agrobacterium tumefaciens. Because the tumor induction involves transfer of a portion of the tumor-inducing (Ti) plasmid DNA from the bacterium to the plant cells, this system is of interest for the study of genetic exchange as well as tumor induction. The boundaries of the transferred DNA (T-DNA) have been cloned from transformed plant cells of tobacco. Detailed mapping with restriction enzymes and nucleotide sequence analysis of two independent clones were used to study the molecular structure of the ends of the T-DNA. One clone contains the two ends of the T-DNA joined together; the other contains one end of the T-DNA joined to repetitive plant DNA sequences. These studies provide direct evidence that the T-DNA can be integrated into the plant genome. In addition, the data suggest that in the plant, T-DNA can be tandemly repeated. Sequence analysis of the junction of crown gall clone 1 reveals several direct repeats as well as an inverted repeat; these structures may be involved in the transfer of the DNA from Agrobacterium to plant cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool.
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Protein secretion and the pathogenesis of bacterial infections.
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The Arabidopsis DELAYED DEHISCENCE1 Gene Encodes an Enzyme in the Jasmonic Acid Synthesis Pathway.
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Introduction of Genetic Material into Plant Cells.
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Crown Gall Disease and Prospects for Genetic Manipulation of Plants.
L. W. Ream and M. P. Gordon (1982)
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