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Science 19 September 1980:
Vol. 209. no. 4463, pp. 1329 - 1336
DOI: 10.1126/science.6158093
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Articles

Science, Vol 209, Issue 4463, 1329-1336
Copyright © 1980 by American Association for the Advancement of Science

articles

Structure and in vitro transcription of human globin genes

NJ Proudfoot, MH Shander, JL Manley, ML Gefter, and T Maniatis

The alpha-like and beta-like subunits of human hemoglobin are encoded by a small family of genes that are differentially expressed during development. Through the use of molecular cloning procedures, each member of this gene family has been isolated and extensively characterized. Although the alpha-like and beta-like globin genes are located on different chromosomes, both sets of genes are arranged in closely linked clusters. In both clusters, each of the genes is transcribed from the same DNA strand, and the genes are arranged in the order of their expressions during development. Structural comparisons of immediately adjacent genes within each cluster have provided evidence for the occurrence of gene duplication and correction during evolution and have led to the discovery of pseudogenes, genes that have acquired numerous mutations that prevent their normal expression. Recently, in vivo and in vitro systems for studying the expression of cloned eukaryotic genes have been developed as a means of identifying DNA sequences that are necessary for normal gene function. This article describes the application of an in vitro transcription procedure to the study of human globin gene expression.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients.
S. Kobayashi, A. Ito, D. Okuzaki, H. Onda, N. Yabuta, I. Nagamori, K. Suzuki, H. Hashimoto, and H. Nojima (2008)
DNA Res 15, 253-265
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A newly discovered human {alpha}-globin gene.
S.-H. Goh, Y. T. Lee, N. V. Bhanu, M. C. Cam, R. Desper, B. M. Martin, R. Moharram, R. B. Gherman, and J. L. Miller (2005)
Blood 106, 1466-1472
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Repression of TFIIH Transcriptional Activity and TFIIH-Associated cdk7 Kinase Activity at Mitosis.
J. J. Long, A. Leresche, R. W. Kriwacki, and J. M. Gottesfeld (1998)
Mol. Cell. Biol. 18, 1467-1476
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Mouse globin system: a functional and evolutionary analysis.
P Leder, J. Hansen, D Konkel, A Leder, Y Nishioka, and C Talkington (1980)
Science 209, 1336-1342
   Abstract »    PDF »


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