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Science 1 August 1980:
Vol. 209. no. 4456, pp. 612 - 615
DOI: 10.1126/science.6248962
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Articles

Science, Vol 209, Issue 4456, 612-615
Copyright © 1980 by American Association for the Advancement of Science

articles

Genetic variation in the human insulin gene

A Ullrich, TJ Dull, A Gray, J Brosius, and I Sures

Four recombinant lambda phages containing nucleotide sequences complementary to a cloned human preproinsulin DNA probe have been isolated from human DNA. Restriction analyses in conjunction with Southern hybridizations reveal two types of gene sequences. One isolate of each type was subjected to complete nucleotide sequence determination. The sequences contain the entire preproinsulin messenger RNA region, two intervening sequence. 260 nucleotides upstream from the messenger RNA capping site, and 35 nucleotides beyond the polyadenylate attachment site. Our results strongly suggest that these two gene types are allelic variants of a single insulin gene.


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