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Science 6 June 1980:
Vol. 208. no. 4448, pp. 1152 - 1155
DOI: 10.1126/science.7189605
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Articles

Science, Vol 208, Issue 4448, 1152-1155
Copyright © 1980 by American Association for the Advancement of Science

articles

In vitro reassembly of squid brain intermediate filaments (neurofilaments): purification by assembly-disassembly

RV Zackroff and RD Goldman

Intermediate filaments from squid brain tissue were reassembled in vitro and purified by two cycles of assembly and disassembly. Purified squid brain filaments contained one major polypeptide (60,000 daltons), which constituted about 70 percent of the total protein, and three minor polypeptides (74,000, 100,000, and 220,000 daltons). Squid brain intermediate filaments were reconstituted from rod-shaped protofilamentous subunits. In addition to the intermediate filaments, dense bodies which may function in intermediate filament nucleation or organization were retained through two purification cycles.


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Chemistry and Biology of Neuronal and Glial Intermediate Filaments.
R. K. H. Liem, C. H. Keith, J. F. Leterrier, E. Trenkner, and M. L. Shelanski (1982)
Cold Spring Harb Symp Quant Biol 46, 341-350
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Heterogeneity of Intermediate Filaments Assembled In Vitro.
P. Steinert, W. Idler, M. Aynardi-Whitman, R. Zackroff, and R. D. Goldman (1982)
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