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Science 30 May 1980:
Vol. 208. no. 4447, pp. 1033 - 1035
DOI: 10.1126/science.6246577
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Articles

Science, Vol 208, Issue 4447, 1033-1035
Copyright © 1980 by American Association for the Advancement of Science

articles

Insertion of a new gene of viral origin into bone marrow cells of mice

KE Mercola, HD Stang, J Browne, W Salser, and MJ Cline

DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a tk++ status in vitro using methotrexate as a selective agent. HSVtk DNA was also used to transform mouse bone marrow cells in vitro. Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences.


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