Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 4 April 1980:
Vol. 208. no. 4439, pp. 57 - 59
DOI: 10.1126/science.6927840
Prev | Table of Contents | Next

Articles

Science, Vol 208, Issue 4439, 57-59
Copyright © 1980 by American Association for the Advancement of Science

articles

Nucleotide sequence of human preproinsulin complementary DNA

I Sures, DV Goeddel, A Gray, and A Ullrich

Recombinant bacterial plasmids that contain DNA complementary to human preproinsulin messenger RNA have been constructed. One clone contains the entire preproinsulin coding region, as well as the 3' untranslated region of the messenger RNA and eight nucleotides of the 5' untranslated region. Additional sequence information for the 5' untranslated region was obtained with the use of insulinoma messenger RNA in conjunction with specific primers from the cloned DNA for enzymatic chain termination sequence analysis. The results confirm the amino acid sequence of human proinsulin previously determined, and predict the amino acid sequence of the human preproinsulin signal peptide.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Allelic Variation in Key Peptide-Binding Pockets Discriminates between Closely Related Diabetes-Protective and Diabetes-Susceptible HLA-DQB1*06 Alleles.
R. A. Ettinger, G. K. Papadopoulos, A. K. Moustakas, G. T. Nepom, and W. W. Kwok (2006)
J. Immunol. 176, 1988-1998
   Abstract »    Full Text »    PDF »
A Peptide Binding Motif for HLA-DQA1*0102/DQB1*0602, the Class II MHC Molecule Associated with Dominant Protection in Insulin-Dependent Diabetes Mellitus.
R. A. Ettinger and W. W. Kwok (1998)
J. Immunol. 160, 2365-2373
   Abstract »    Full Text »    PDF »
Genetic variation in the human insulin gene.
A Ullrich, T. Dull, A Gray, J Brosius, and I Sures (1980)
Science 209, 612-615
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)