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Science 28 March 1980:
Vol. 207. no. 4438, pp. 1479 - 1481
DOI: 10.1126/science.7361100
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Articles

Science, Vol 207, Issue 4438, 1479-1481
Copyright © 1980 by American Association for the Advancement of Science

articles

Cytosolic and microsomal epoxide hydrolases: differential properties in mammalian liver

K Ota and BD Hammock

The epoxide hydrolase activities of the 100,000 g pellet (microsomal) and 100,00 g soluble (cystosolic) fractions of mouse, rat, and guinea pig liver were measured with three closely related compounds used as substrates. Differences between the species in the distribution of the cytosolic and microsomal hydrolases and in their substrate specificities and pH optima demonstrate why epoxide hydrolase activity in the cytosolic fraction was not detected earlier in spie of intensive work on the microsomal epoxide hydrolase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Hydrolysis of cis- and trans-Epoxyeicosatrienoic Acids by Rat Red Blood Cells.
H. Jiang, A. G. Zhu, M. Mamczur, C. Morisseau, B. D. Hammock, J. R. Falck, and J. C. McGiff (2008)
J. Pharmacol. Exp. Ther. 326, 330-337
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Distribution and Expression of Soluble Epoxide Hydrolase in Human Brain.
P. Sura, R. Sura, A. E. EnayetAllah, and D. F. Grant (2008)
J. Histochem. Cytochem. 56, 551-559
   Abstract »    Full Text »    PDF »
Distribution of Soluble Epoxide Hydrolase and of Cytochrome P450 2C8, 2C9, and 2J2 in Human Tissues.
A. E. Enayetallah, R. A. French, M. S. Thibodeau, and D. F. Grant (2004)
J. Histochem. Cytochem. 52, 447-454
   Abstract »    Full Text »    PDF »
Epoxide Hydrolases in the Rat Epididymis: Possible Roles in Xenobiotic and Endogenous Fatty Acid Metabolism.
S. B. DuTeaux, J. W. Newman, C. Morisseau, E. A. Fairbairn, K. Jelks, B. D. Hammock, and M. G. Miller (2004)
Toxicol. Sci. 78, 187-195
   Abstract »    Full Text »    PDF »
The N-terminal domain of mammalian soluble epoxide hydrolase is a phosphatase.
A. Cronin, S. Mowbray, H. Durk, S. Homburg, I. Fleming, B. Fisslthaler, F. Oesch, and M. Arand (2003)
PNAS 100, 1552-1557
   Abstract »    Full Text »    PDF »
Detoxification of environmental mutagens and carcinogens: Structure, mechanism, and evolution of liver epoxide hydrolase.
M. A. Argiriadi, C. Morisseau, B. D. Hammock, and D. W. Christianson (1999)
PNAS 96, 10637-10642
   Abstract »    Full Text »    PDF »
Asp[IMAGE], Asp[IMAGE], and His[IMAGE] Form the Catalytic Triad of Rat Soluble Epoxide Hydrolase.
M. Arand, H. Wagner, and F. Oesch (1996)
J. Biol. Chem. 271, 4223-4229
   Abstract »    Full Text »    PDF »
Subcellular Distribution of Styrene Oxide in Rat Liver.
G. M. Pacifici, L. Cuoci, and A. Rane (1984)
Toxicol Pathol 12, 69-73
   Abstract »    PDF »
Detoxification Enzyme Differences Between a Herbivorous and Predatory Mite.
C. A. MULLIN, B. A. CROFT, K. STRICKLER, F. MATSUMURA, and J. R. MILLER (1982)
Science 217, 1270-1272
   Abstract »    PDF »
Binding of Alkylurea Inhibitors to Epoxide Hydrolase Implicates Active Site Tyrosines in Substrate Activation.
M. A. Argiriadi, C. Morisseau, M. H. Goodrow, D. L. Dowdy, B. D. Hammock, and D. W. Christianson (2000)
J. Biol. Chem. 275, 15265-15270
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)