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Science 28 March 1980:
Vol. 207. no. 4438, pp. 1475 - 1477
DOI: 10.1126/science.7361099
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Articles

Science, Vol 207, Issue 4438, 1475-1477
Copyright © 1980 by American Association for the Advancement of Science

articles

Role of nitrogen dioxide in the biosynthesis of nitraosamines in mice

ZM Iqbal, K Dahl, and SS Epstein

Groups of three to four mice were gavaged with aqueous solutions of 2 milligrams of morpholine, after which they were exposed to nitrogen dioxide in inhalation chambers at concentrations of 0.2 to 50 parts per million for up to 4 hours. At sequential intervals during the exposure, mice were frozen and pulverized in liquid nitrogen, and the mice powder was extracted with ice-cold 35 percent aqueous methanol and dichloromethane; organic-phase concentrates were analyzed for N-nitrosomorpholine with a thermal energy analyzer interfaced to a gas chromatograph. The N-nitrosomorpholine yields, ranging up to about 2.3 micrograms per mouse, were time-dependent relative to the duration of exposure to nitrogen dioxide and dose-dependent relative to the concentrations of nitrogen dioxide; control levels (in mice that were gavaged with morpholine or distilled water and then exposed to air instead of nitrogen dioxide) were less than 5 nanograms per mouse. These preliminary studies demonstrate the in vivo nitrosating potential of nitrogen oxides.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Repeated Measurements of Urinary Methylated/Oxidative DNA Lesions, Acute Toxicity, and Mutagenicity in Coke Oven Workers.
M.-R. Chao, C.-J. Wang, M.-T. Wu, C.-H. Pan, C.-Y. Kuo, H.-J. Yang, L. W. Chang, and C.-W. Hu (2008)
Cancer Epidemiol. Biomarkers Prev. 17, 3381-3389
   Abstract »    Full Text »    PDF »
6 Final Report on the Safety Assessment of Morpholine.
(1989)
International Journal of Toxicology 8, 707-748
   Abstract »    PDF »
5 Addendum to the Final Report on the Safety Assessment of 2-Bromo-2-Nitropropane-1,3-Diol.
(1984)
International Journal of Toxicology 3, 139-155
   Abstract »    PDF »
Gut reactions of radioactive nitrite after intratracheal administration in mice.
Thayer JR, J. Chasko, L. Swartz, and N. Parks (1982)
Science 217, 151-153
   Abstract »    PDF »
Mechanisms of Nitrogen Dioxide Reactions: Initiation of Lipid Peroxidation and the Production of Nitrous Acid.
W. A. PRYOR and J. W. LIGHTSEY (1981)
Science 214, 435-437
   Abstract »    PDF »
Nitrogen-13-labeled nitrite and nitrate: distribution and metabolism after intratracheal administration.
N. Parks, K. Krohn, C. Mathis, J. Chasko, K. Geiger, M. Gregor, and N. Peek (1981)
Science 212, 58-60
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)