Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 March 1980:
Vol. 207. no. 4436, pp. 1218 - 1220
DOI: 10.1126/science.7355284
Prev | Table of Contents | Next

Articles

Science, Vol 207, Issue 4436, 1218-1220
Copyright © 1980 by American Association for the Advancement of Science

articles

Babesia bovis: continuous cultivation in a microaerophilous stationary phase culture

MG Levy and M Ristic

The protozoan parasite Babesia bovis, a causative agent of bovine babesiosis, has been continuously cultivated in a settled layer of bovine erythrocytes. Lowered oxygen tension within the layer of host erythrocytes results in a darkening of infected cultures and provides a rapid means of evaluating parasite growth. Deprivation of carbon dioxide causes the merozoites to accumulate in the medium rather than involving new erythrocytes. When separated from the culture, these extraerythrocytic parasites retain their infectivity. Parasites produced in vitro are morphologically identical to parasites from the blood of infected cattle and are susceptible to antibabesial drugs.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Development of a Rapid Immunochromatographic Test for Simultaneous Serodiagnosis of Bovine Babesioses Caused by Babesia bovis and Babesia bigemina.
C.-M. Kim, L. B. C. Blanco, A. Alhassan, H. Iseki, N. Yokoyama, X. Xuan, and I. Igarashi (2008)
Am J Trop Med Hyg 78, 117-121
   Abstract »    Full Text »    PDF »
Development of Taqman-Based Real-Time PCR Assays for Diagnostic Detection of Babesia bovis and Babesia bigemina.
C. Kim, H. Iseki, M. S. Herbas, N. Yokoyama, H. Suzuki, X. Xuan, K. Fujisaki, and I. Igarashi (2007)
Am J Trop Med Hyg 77, 837-841
   Abstract »    Full Text »    PDF »
Immunologic and Molecular Identification of Babesia bovis and Babesia bigemina in Free-Ranging White-Tailed Deer in Northern Mexico.
A. Cantu, J. A. Ortega-S, J. Mosqueda, Z. Garcia-Vazquez, S. E. Henke, and J. E. George (2007)
J. Wildl. Dis. 43, 504-507
   Abstract »    Full Text »    PDF »
Sequence Variation and Immunologic Cross-Reactivity among Babesia bovis Merozoite Surface Antigen 1 Proteins from Vaccine Strains and Vaccine Breakthrough Isolates.
T. LeRoith, K. A. Brayton, J. B. Molloy, R. E. Bock, S. A. Hines, A. E. Lew, and T. F. McElwain (2005)
Infect. Immun. 73, 5388-5394
   Abstract »    Full Text »    PDF »
In Vitro Cultivation of a Zoonotic Babesia sp. Isolated from Eastern Cottontail Rabbits (Sylvilagus floridanus) on Nantucket Island, Massachusetts.
P. J. Holman, A. M. Spencer, R. E. Droleskey, H. K. Goethert, and S. R. Telford III (2005)
J. Clin. Microbiol. 43, 3995-4001
   Abstract »    Full Text »    PDF »
Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity.
N. Yokoyama, B. Suthisak, H. Hirata, T. Matsuo, N. Inoue, C. Sugimoto, and I. Igarashi (2002)
Infect. Immun. 70, 5822-5826
   Abstract »    Full Text »    PDF »
Cultivation of Babesia and Babesia-Like Blood Parasites: Agents of an Emerging Zoonotic Disease.
F. L. Schuster (2002)
Clin. Microbiol. Rev. 15, 365-373
   Abstract »    Full Text »    PDF »
The Babesia bovis Merozoite Surface Antigen 2 Locus Contains Four Tandemly Arranged and Expressed Genes Encoding Immunologically Distinct Proteins.
M. Florin-Christensen, C. E. Suarez, S. A. Hines, G. H. Palmer, W. C. Brown, and T. F. McElwain (2002)
Infect. Immun. 70, 3566-3575
   Abstract »    Full Text »    PDF »
Characterization of Allelic Variation in the Babesia bovis Merozoite Surface Antigen 1 (MSA-1) Locus and Identification of a Cross-Reactive Inhibition-Sensitive MSA-1 Epitope.
C. E. Suarez, M. Florin-Christensen, S. A. Hines, G. H. Palmer, W. C. Brown, and T. F. McElwain (2000)
Infect. Immun. 68, 6865-6870
   Abstract »    Full Text »    PDF »
Cytoadherence of Babesia bovis-Infected Erythrocytes to Bovine Brain Capillary Endothelial Cells Provides an In Vitro Model for Sequestration.
R. M. O'Connor, J. A. Long, and D. R. Allred (1999)
Infect. Immun. 67, 3921-3928
   Abstract »    Full Text »    PDF »
In Vivo Binding of Immunoglobulin M to the Surfaces of Babesia bigemina-Infected Erythrocytes.
I. E. Echaide, S. A. Hines, T. F. McElwain, C. E. Suarez, T. C. McGuire, and G. H. Palmer (1998)
Infect. Immun. 66, 2922-2927
   Abstract »    Full Text »    PDF »
Bovine babesiosis: protection of cattle with culture-derived soluble Babesia bovis antigen.
R. Smith, M. James, M Ristic, M Aikawa, and y C. Vega Murguia (1981)
Science 212, 335-338
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)